商品描述:
商品属性
商品描述:
商品属性
苹果酸脱氢酶
中文名称: 苹果酸脱氢酶
英文名称: MDH
其他英文名称: Malate Dehydrogenase;Malic Dehydrogenase from porcine heart
产品规格: 超纯,1200u/mg,液体
产品规格: BR,200u/mg,粉末
包装: 25KU
CAS号:9001-64-3
级别:Ultra Pure Grade
活力:≥1200units/mg protein
苹果酸脱氢酶活力定义:One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25℃
Concentration:>12000u/ml
Alanine Aminotransferase:≤0.005%
Aspartate Aminotransferase:≤0.005%
Lactate Dehydrogenase:≤0.005%
分子量:34KDa(SDS-PAGE)
级别:BR
活力:200units/mg protein
活力定义:One unit will cause the oxidation of one micromole NADH per min at PH7.5 at 30℃
最佳PH:9.0
PH稳定性:4.0~8.0
最佳温度:55℃
Thermal stability:<60℃(PH 7.5, 1.5min)
抑制剂:Ag+,Hg2+
Preparation Instructions:The enzyme is reconstituted in 100mM potassium phosphate buffer,PH 7.5 containing 0.2% BSA for activity assay
性状:白色无定形粉末,Isoelectic point:4.9, 溶解时候用浓度50毫摩尔的磷酸钾缓冲液,PH在6-8之间,可以加进去一点0.2%的BSA
苹果酸脱氢酶性状:悬浮液,分子量约70000,等电点PH6.1~6.4
用途:生化研究。还原草酰乙酸成为苹果酸或催化苹果酸脱氢生成草酰乙酸
保存:2~8℃