荧光素酶

荧光素酶
中文名称： 荧光素酶 
其他中文名称： 荧光素酶(萤火虫)；重组型酵素 
英文名称： Luciferase firefly 
其他英文名称： Luciferase from Photinus pyralis (firefly) 
产品规格： BR，98% 
包装： 1毫克 
CAS号：61970-00-1
级别：BR
product line：BioUltra
荧光素酶Assay：≥98% (SDS-PAGE)
Mol wt：mol wt 120 kDa (two non-identical subunits, each containing four free thiol groups, one of which is necessary for activity)
Protein：10-35% E1%/280
Measuring range：≤1 femtomole ATP sensitivity, (using 0.2 μg of luciferase and suitably sensitive liquid scintillation counters or luminometers)
Foreign activity：ATPase ≤5 nanomolar units/mg protein；Nucleoside diphosphokinase ≤20 nanomolar units/mg protein
活力定义：One light unit produces a biometer peak height equivalent to 0.02 μCi of 14C in PPO/POPOP cocktail. Light units measured in 50 μl assay mixture containing 5 pmol ATP and 7.5 nmol luciferin in Tris-glycine buffer, pH 7.6, at 25 °C
产品描述：Two contaminant, ATP-consuming activities are assayed for in this product, ATPase and nucleoside diphosphokinase. These impurities are found to be less than 5 nanomolar units/mg protein and less than 20 nanomolar units/mg protein, respectively. 
Note: Prior to 1991, a unit of firefly luciferase activity was defined as that amount which will produce 1.0 nanomole of pyrophosphate per minute at pH 7.7, 25 °C, using a system containing 0.6 mM ATP and 0.1 mM D-luciferin. The former nanomolar unit is equivalent to approximately 1.3 x 106 light units
性状：粉末。EC：1.13.12.7
用途：生化研究。
荧光素酶保存：-20℃。保质期1年