磷酸二酯酶I

磷酸二酯酶I
中文名称： 磷酸二酯酶I
其他中文名称： 磷酸二脂酶；磷酸二酯酶
英文名称： Phosphodiesterase I from Crotalus adamanteus venom
其他英文名称： 5′-Exonuclease；Oligonucleate 5′-nucleotidohydrolase
产品规格： BR，20u/mg
包装： 100U
CAS号：9025-82-5
磷酸二酯酶I级别：BR
Activity：≥20u/mg
活力定义：One Unit hydrolyzes one micromole of p-nitrophenyl thymidine-5-phosphate per minute at 25℃, pH 8.9.
产品描述：Venom exonuclease (Phosphodiesterase I) successively hydrolyzes 5'-mononucleotides from 3'-OH-terminated ribo- and deoxyribo-oligonucleotides. The enzyme has an optimal pH range of 9.8-10.4 and a molecular weight of 115 kDa. Phosphodiesterase is inhibited by reducing agents such as glutathione, cysteine and ascorbic acids. It is completely inhibited by 5mM EDTA while ATP, ADP and AMP are partial inhibitors. The enzyme has an absolute requirement for Mg2+
性状：白色或类白色粉末
用途：生化研究。
磷酸二酯酶I保存：-20℃