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磷酸二酯酶Ⅱ
中文名称: 磷酸二酯酶Ⅱ
其他中文名称: 磷酸二脂酶;磷酸二酯酶
英文名称: Phosphodiesterase II from bovine spleen
其他英文名称: 3′-Exonuclease;Spleen exonuclease;Spleen phosphodiesterase
产品规格: BR,10u/mg
包装: 10U
CAS号:9068-54-6
级别:BR
Activity:≥10u/mg
磷酸二酯酶Ⅱ活力定义:One Unit increases the absorbance at 260 nm by 0.200 in 30 minutes at 37℃, pH 6.5, with an RNA substrate
产品描述:Spleen exonuclease (Phosphodiesterase II) excises 3'-phosphomononucleotides from oligonucleotides having a free OH terminus. The optimum pH for the enzyme is 5.5 using succinate and phosphate buffer and pH 6-7 with 0.1 M acetate buffer. The enzyme is assayed using a modification of the Hilmoe (Biochem. Prep., VII, (Meister, A., ed.), John Wiley and Sons, NY, 105, 1961) technique
性状:粉末
用途:生化研究。
磷酸二酯酶Ⅱ保存:-20℃
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